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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has long been noticed in many tumors, which results in chromosome instability, and so may possibly lead to tumorigenesis. This protein was discovered to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a significant position in marketing cell-cycle development within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, have been explained. Two extra splice variants were being documented but comprehensive nucleotide sequence facts just isn't yet offered.
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Blocking peptides are peptides that bind precisely towards the focus on antibody and block antibody binding. These peptide ordinarily includes the epitope identified by the antibody.
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This manual outlines additional possible optimization of mobile and immunoassay parameters to guarantee the absolute best outcomes are...
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This tutorial outlines further more probable optimization of mobile and immunoassay parameters to ensure the very best results are...
Antibodies sure to the blocking peptide not bind for the epitope within the goal protein. By comparing the staining from the blocked antibody vs . the antibody by yourself, one can see which staining is unique.
Concentrate on Facts Cyclin E belongs for the really conserved cyclin loved ones, whose associates are characterized by a remarkable periodicity in protein abundance with the mobile cycle. Cyclins purpose as regulators of CDK kinases. Unique cyclins exhibit unique expression and degradation designs which contribute for the temporal coordination of every mitotic event. Cyclin E forms a posh with and features as a regulatory subunit of CDK2, whose exercise is needed for mobile cycle G1/S transition. This protein accumulates in the G1-S section boundary and is also degraded as cells progress by S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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